
Lemonade stand is a great experience
Justin is a 7-year-old who enjoys playing baseball and soccer. He will enter second grade at Dingeman Elementary School this year.
“A few weeks ago, we saw some kids selling lemonade at the top of our street and we stopped,” Julie explained. “After that, he was very curious and asked lots of questions about it. I told him we could do one sometime and he talked about it often.”

Believe it or not, this was the first lemonade stand for both. Julie never had the opportunity as a child, so the mother-son business venture was special. Julie baked double chocolate chip and regular chocolate chip cookies most of the morning on Saturday. She said she didn’t want to simply offer lemonade, and it turns out the cookies were a big hit.
In addition, Julie created some signs to get the attention of potential customers.
“I love doing crafts, so this presented me with an opportunity to get crafty and I got a bit carried away with the signs,” she said. “I got lots of compliments on them, though, so that was nice.”

The two partners opened for business at 11:30 a.m. Sunday in the hot sun.
Justin was pretty nervous getting out there with his sign, but once customers started stopping, talking with the two entrepreneurs and putting money in his jar, he became very excited, Julie said.
“We’re rockin’ it, Mommy!” he exclaimed.
At one point, two convertibles stopped by, one after the other, and the business team served them at their car; curbside-service-style. The two also met some neighbors who live on their street. A few familiar faces came to visit and contributed to their business as well.
“It was a great experience for both of us,” Julie said.
The pair put in a good three hours under the summer’s sun, and closed shop at 2:30 p.m. Both were hot and tired by that time, but happy to have enjoyed a good day of business, according to Julie.
“It was actually a perfect time to do it, too,” Julie explained. Justin had been asking for a “Cars 3” racing track for his die-cast cars, and profits from his lemonade stand made that wish a reality.


Lemonade stands, kids and families are what Scripps Ranch News is all about. No story is too big or too small. Please contact us with community announcements about similar projects, especially lemonade stands. Send photos and information to: john@scrippsranchnewsws.com.

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Since irritation is a stability between pro- and anti inflammatory cytokines, we also assessed the impact of KPV on the main anti-inflammatory cytokine IL-10. We discovered that KPV did not change IL-10 mRNA expression in mouse colon (data not shown) suggesting that KPV acts by reducing pro-inflammatory cytokines quite than increasing anti-inflammatory cytokines. Figure 3E reveals that in distinction to KPV, Gly-Leu didn’t affect IL-1β-induced activation of NF-κB demonstrating the specificity of the KPV impact. Nonetheless, KPV-mediated lower of IL-1β-induced activation of NF-κB was completely reversed by the addition of Gly-Leu. To additional confirm this end result, we used HT29-Cl.19A cells beforehand stably transfected with hPepT1 or empty vector (17). These cells have been transiently transfected with a NF-κB-dependent luciferase reporter plasmid and handled with the abovementioned stimuli.
It is known that MAPK and NF-κB pathways activation in IEC induces the manufacturing of pro-inflammatory cytokines that have a role within the recruitment of immune cells corresponding to IL-8 (25). To look at whether KPV affects IL-8 expression and secretion by Caco2-BBE cells, IL-8 mRNA and protein levels have been assessed by real-time RT-PCR and ELISA. We discovered that IL-1β induced a ~200-fold increase of IL-8 mRNA after 3 hours of stimulation in comparison with untreated cells (Figure 1E). In the presence of KPV, nonetheless, the IL-1β-induced improve of IL-8 mRNA was considerably lowered (by ~35%) (Figure 1E).
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Examination of the cells indicated that even nanomolar concentrations of the peptide appeared to produce anti-inflammatory results. Researchers advised that the peptide’s action might be mediated via PepT1 expression in these intestinal cells, implicating PepT1 in transporting the peptide to inflammation websites. Zhang et al. (2024) reported breakthrough findings in cardiovascular functions through carrier-free nanodrug growth.
KPV-mediated decrease of NF-κB activity was also confirmed by EMSA (Supplementary outcomes, Figure 1). KPV peptide is primarily used to minimize back irritation, enhance intestine health, and promote wound healing. Ongoing analysis is investigating how KPV peptide can be utilized to a broader range of situations. Early indications counsel that it might be helpful in treating neurodegenerative illnesses, persistent pain, and even certain types of cancer as a outcome of its anti-inflammatory and immune-modulating properties.
Their study incorporated the peptide alongside other bioactive factors in superior supply methods that demonstrated outstanding efficacy in repairing mucosal obstacles. The analysis revealed important enhancements in colonic epithelium restoration and tight junction protein expression, establishing new approaches for gastrointestinal therapeutic purposes. One Other study investigated the anti-inflammatory results of KPV in TNBS-induced colitis models. The results showed that KPV considerably decreased weight reduction, MPO exercise, and pro-inflammatory cytokine levels in the colon. These findings had been further supported by real-time RT-PCR analyses, confirming the tripeptide’s capability to mitigate irritation in vivo. KPV acts via the hPepT1 transporter, which is expressed in each immune and intestinal epithelial cells.
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